Ukuphucula uKhetho lweGenomic nge-KASP Low Density Genotyping Platform
Njengoko inkqubela phambili kwi-genotyping kunye netekhnoloji yokulandelelanisa iqhubeka nokutshintsha ezolimo, ukusetyenziswa kweempawu ze-molecular ezisekelwe kwi-DNA kuye kwaba yinto ebalulekileyo kwiinkqubo zokuphucula izityalo. Ezi mpawu, ezibekwe ngokwesicwangciso kwi-genome, zibonelela ngengqiqo exabisekileyo kwiimpawu ezibalulekileyo kwezolimo, zinika amandla abafuyi ukuba bakhawulezise uphuhliso lweentlobo zezityalo ezibalaseleyo.
Ukwazisa iqonga le-genotyping eliphantsi le-Kompetitive Allele Specific PCR (KASP), isisombululo esiphambili esinikezelwa yi-EiB Genotyping Services. Itekhnoloji ye-KASP, eyaziwa ngokulula kwayo nokusebenza kakuhle, ivumela i-genotyping ekhawulezayo nengabizi kakhulu yee-polymorphisms ezithile okanye ii-INDELS (ukufakwa/ukususwa). Eli qonga, elisebenza phakathi kukaDisemba 2021 noJanuwari 2022, libonelela ngezicelo ezahlukeneyo kubandakanya ukuhlolwa kweempawu, ulawulo lomgangatho, kunye nokukhetha okuncediswa yi-marker (MAS).
Ukuguquguquka kweqonga le-KASP kuxhomekeke ekubeni likwazi ukwamkela phakathi kwe-1 kunye ne-200 markers, nto leyo eyenza ukuba libe lolona khetho lufanelekileyo kwiiprojekthi ezifuna i-genotyping echanekileyo nejolise kuyo. Ngokusebenzisa amandla e-KASP markers, abafuyi banokukhawulezisa ukuchonga ii-alleles ezilungileyo ezinxulumene neempawu ezifunwayo, nto leyo eququzelela ukukhethwa kwezinto zokufuya ezikumgangatho ophezulu.
Iintsuku zeveki. Zikhuphe kubhaliso ngokucofa kube kanye.
Kwabo baqalisa uhambo lwabo lokuchonga i-genotyping, kubalulekile ukuhlala benolwazi ngoluhlu oluhlala lutshintsha lweempawu ezikhoyo. Iinkonzo zokuchonga i-EiB Genotyping ziqinisekisa ukuba olu luhlu luhlala lutshintsha kwaye lubanzi, lubonisa inkqubela phambili yamva nje kuphando lwe-genomic. Abasebenzisi abatsha bayakhuthazwa ukuba badibane neengcali ze-EiB ukuze balungelelanise amaqhinga abo okuchonga i-genotyping ukuze bafumane iziphumo ezilungileyo.
Kwixesha elichazwe bubuchule kunye nolimo oluchanekileyo, iqonga le-KASP lokuchonga i-genotyping eliphantsi livela njengophawu lokusebenza kakuhle, linika amandla abafuyi ukuba bavule amandla apheleleyo okwahlukana kwezakhi zofuzo kwimizamo yokuphucula izityalo.
https://excellenceinbreeding.org/toolbox/tools/kasp-low-density-genotyping-platform
